Transformation of Escherichia coli by chromosomal deoxyribonucleic acid from Salmonella typhi.
نویسندگان
چکیده
The genetic transformation of Escherichia coli K-12 with chromosomal deoxyribonucleic acid purified from Salmonella typhi is described.
منابع مشابه
Cloning and sequencing of ompf Salmonella typhi Salmonella ompf gene in Escherichia coli Origami
Background and Aim: Salmonella Typhi belongs to the family Enterobacteriaceae, gram-negative bacilli and causes gastrointestinal diseases such as typhoid. This bacterium has a special structure and various genes, including the ompf gene (outer membrane protein). Recent studies have shown the possibility of using ompf in the development of a diagnostic tuberculosis vaccine. Therefore, the aim of...
متن کاملبررسی اثر ضد باکتریایی عصاره آبی گزنه و مرزنگوش بر Escherichia coli، Salmonella typhi و Pseudomonas aeruginosa
فعالیت ضدباکتریایی (حداقل غلظت مهارکنندگی و کشندگی) عصاره آبی گزنه و مرزنگوش در مقابل 3 باکتری بیماریزا و عامل فساد مواد غذایی Escherichia coli, Salmonella typhi و Pseudomonasaeruginosa با استفاده از روش رقتلولهای(Macro Dilution Method) ارزیابی شد. مقدار حداقل غلظت بازدارندگی عصاره آبی مرزنگوش بر Salmonella typhi mg/ml 25/1 و در مورد Pseudomonas aeruginosaو Escherichia coli برابر با mg/ml 5 ...
متن کاملIdentification of a new iron regulated locus of Salmonella typhi.
In order to identify genes belonging to the Fur regulon of Salmonella typhi which are absent from Escherichia coli K-12, a plasmid gene bank consisting of 4000 independent clones was screened for Fur regulated promoters using the Fur titration assay (FURTA). DNA probes generated from FURTA positive plasmids were then used for hybridization with chromosomal DNA from S. typhi, Salmonella typhimur...
متن کاملTransformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristics of the transforming plasmid.
متن کاملChemical Composition, Antioxidant Activity and Antimicrobial Effect of Rosa damascena Mill. Essential Oil Against Staphylococcus aureus, Escherichia coli and Salmonella typhi
Food preservation is an action or a method of maintaining foods at a desired level of properties or nature for their maximum benefits. In general, each step of handling, processing, storage, and distribution affects the characteristics of food, which may be desirable or undesirable. Thus, understanding the effects of each preservation method and handling procedure on foods is critical in food p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of bacteriology
دوره 125 2 شماره
صفحات -
تاریخ انتشار 1976